Creatine Kinase: ATP Contaminating Creatine Phosphate Substrate of a Commercial Kit

Creatine kinase: ATP contaminating creatine phosphate substrate of a commercial kit.

Creatine Kinase Activity and Cartilage Energy Metabolism: Creatine Kinase-creatine Phosphate Circuit Activity and Macromolecule Synthesis

Earlier studies showed that there is significant creatine kinase activity in the epiphyseal growth cartilage, and there is stage-specific isoform expression. To further explore the role of this enzyme in cartilage development, we disrupted the creatine kinase creatine phosphate (CK-CP) circuit using the creatine analogue, cyclocreatine. We found that while cyclocreatine decreased creatine kinas...

748 Creatine Kinase and Creatine Phosphate in Macrophages

Creatine phosphate serves as a reuseable high energy phosphate reservoir in skeletal and cardiac muscle and in the brain. These stores of creatine phosphate are thought to rephosphorylate the ATP hydrolyzed during cellular work (see 1 and 2 for review). Studies in our laboratory (3) have shown that mononuclear phagocytes obtained from the peritoneal cavities of mice also contain large stores of...

NOVEL REAGENTS FOR CREATINE KINASE

The purpose of this investigation was to develop a simple colorimetric method for creatine kinase (CK). The new method is based on the reaction of creatine, formed enzymatically from creatine phosphate and ADP, with different glyoxal compounds. Hydrated glyoxals, such as para-nitrophenyl, 2-thiophene, 4- biphenyl, 4, 4' -biphenyl,α-naphthyl, β-naphthyl, para-chlorophenyl, and styryl were...

Creatine-creatine phosphate shuttle modeled as two-compartment system at different levels of creatine kinase activity.

In order to characterize ADP-ATP and creatine-creatine phosphate (Cr-CrP) shuttles a minimal mathematical model with two compartments and cyclic turnover of matter was designed. The 'mitochondrial' compartment contained 'ATP-synthase' and 'mitochondrial creatine kinase' (mitCK). The 'cytoplasmic' compartment consisted of 'ATPase', 'cytoplasmic creatine kinase' (cytCK) and an 'ADP-binding struct...